Protein Purification

• Purification of LuxR : Protocol and Results
• Purification of GFPmut3b: Protocol and Results

Fluorometer Testing

• Optimisation of Fluorometer: Protocol and Results

Calibration Curve

• Calibration Curve for GFPmut3b Protocol and Results
• Degradation Curve for GFPmut3b Protocol and Results

Chassis Protocols

• Preparation S30 Cell Extract Protocol
• Preparation S12 Cell Extract Protocol
• Preparation of Reaction Mixture Protocol
• Vesicles Formation Vesicles Formation

Cell by Date
Implementation

• In Vivo Testing: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
• Initial In vitro Testing:Protocols
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]

Testing/Validation

• Operating Temperature Range (Initial Tests)
• Operating Temperature Range (Refined Tests)
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.1.1 Results + ones for initial operating range]
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results for pT7]
• Detemining System Capabilities - DNA concentration
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
• Packaging protocol?? Results are in OWW.
• Steps protocol?? Results are in OWW.

Infector Detector
Implementation

• Initial Testing for DNA constructs of ID In Vivo
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
• Initial Testing for DNA constructs of ID In Vitro (Chassis)
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]

Testing/Validation

• Final AHL Testing
• Testing of Purified LuxR
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/Construct_2 Results]