From 2007.igem.org
Wet Lab: Protocols
Below is a databank of all the protocols that have been used in our projects.
| General Experiments | Testing Experiments
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Protein Purification
- Purification of LuxR : Protocol and Results
- Purification of GFPmut3b: Protocol and Results
Fluorometer Testing
- Optimisation of Fluorometer: Protocol and Results
Calibration Curve
- Calibration Curve for GFPmut3b: Protocol and Results
- Degradation Curve for GFPmut3b :Protocol and Results
Chassis Protocols
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pT7 Characterisation
- pT7 in vitro: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results]
Cell by Date
Implementation
- In Vivo Testing: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
- Initial In vitro Testing:Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
Testing/Validation
- Testing DNA Concentration in vitro:
- Operating Temperature Range :Protocol 1 and Protocol 2 and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
- Temperature Steps: Protocols and Results
Infecter Detector
Implementation
- In vivo Testing: Protocol and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
- Initial In Vitro Testing: Protocol and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]
Testing/Validation
- Testing of Purified LuxR :Protocol and Results
- Characterization in vitro - AHL titration : Protocol and Results
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