Imperial/Wet Lab/Protocols

From 2007.igem.org

< Imperial | Wet Lab

Revision as of 23:09, 25 October 2007 by Maira (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments

Testing Experiments

Protein Purification

Purification of LuxR: Protocol and Results
Purification of GFPmut3b: Protocol and Results

Fluorometer Testing

Optimisation of Fluorometer: Protocol and Results

Calibration Curve

Calibration Curve for GFPmut3b: Protocol and Results
Degradation Curve for GFPmut3b:Protocol and Results

Chassis Protocols

Commercially available S30 Cell Extract :Protocol
Homemade S30 Cell Extract :Protocol
Preparation S30 Cell Extract :Protocol
Preparation S12 Cell Extract :Protocol
Vesicles Formation:Protocol

pT7 Characterisation

pT7 in vitro: Protocol and Results

Cell by Date
Implementation

In Vivo Testing: Protocols and Results
Initial In vitro Testing: Protocols and Results

Testing/Validation

Testing DNA Concentration in vitro: Protocols and Results
Isothermal experiments: Protocols and Results
Temperature Steps: Protocol and Results
Packaging experments: Protocols and Results

Infecter Detector
Implementation

In vivo Testing: Protocol and Results
Initial In Vitro Testing: Protocol and Results

Testing/Validation

Testing of DNA concentration : Protocol and Results
Testing of Purified LuxR: Protocol and Results
Characterization in vitro - AHL titration : Protocol and Results

Retrieved from "http://2007.igem.org/wiki/index.php/Imperial/Wet_Lab/Protocols"