From 2007.igem.org

Contents 1 Team Members: 2 Our Project: 3 Support: 4 Lab-Work: 5 Protocols:

Team Members:

Instructors:

Kristian M. Müller, Katja M.Arndt

Students:

Maximilian Mauler

Moritz Busacker

Corinna Gruber

Natalia Maier

Hermida Aponte

Philipp Mappes

Graduate Student:

Dinah Mattay

Advisors:

Andreas Hiltbrunner, Michael Reth, Bodo Rak

Our Project:

Our goal is to design integrated molecular sensing and executing devices based on modular protein engineering. These integrated devices can then easily be used for the construction of complex systems. We plan to fuse sensing proteins, which provide nano-mechanical movements upon an external signal, to executing proteins, which depend in their activity on the nano-mechanical movement of the sensing protein. To elucidate the possibilities of such a system we plan to fuse each of the termini of Calmodulin, which senses Ca2+, or the Maltodextrin Binding Protein, which senses maltodextrin, to one half of a split enzyme such as β-lactamase. We expect that we can design the separation of the two enzyme fragments by one conformation of the sensing protein such that there is no activity and that a conformational change in the sensor will bring the enzyme fragments together resulting in activity of the execution part. Once the basis of the interconnection of sensor and executor has been established, we plan to extend the work in a modular fashion to further proteins in order to generate a readily available and easy to tailor toolbox. We see application of such tools as varied as bacterial growth regulation and tumor targeting.

Support:

[http://www.syntheticbiology.ethz.ch/synbiocomm/index SYNBIOCOMM]

[http://www.uni-freiburg.de/ Universität Freiburg]

Lab-Work:

lab notes
plasmids
PCR primers
Lab Boxes

Protocols:

DNA sequencing
Plasmid spin column prep
Glycerol stocks