From 2007.igem.org

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Cell-Free: Our Contribution

In vitro Expression

• Attempted to make S30 E. coli cell extract and feeding solution
• Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
• Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
• Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution

Vesicles Formation

• Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
• Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
• Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
• Attempting to find suitable pore proteins to prolong vesicle and expression lifespan

Documentation on Registry of Parts

Documented our findings with the following Registry Additions:

• 1
• 2
• 3

<< Cell-Free vs. Cell | Our Contribution | Characterisation >>