From 2007.igem.org

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Infector Detector: Specifications

The system must be able to detect the presence of biofilms on urinary catheters by detection of AHL, at a minimum concentration of 5nM, and report with a visual signal within 3 hours. It must work within a temperature range of 20°-30°C, be portable and easy to use, have a shelf life of at least seven days, and must not be harmful or infectious.

Property	Value
Inputs	System must be sensitive to AHL concentration between 5-50nM
Outputs	System must give a visual signal if bacteria is present
Response Time	System needs to have a response time under 3 hour
Operating Conditions	System must operate within temperature 20-30°C
	System must operate within pH range of 6-8
Health & Safety	System Must not be living replicating bacteria, and in any way harmful or infectious.
Lifespan	System must have a shelf life of 7 days
Packaging	System must be portable and convenient to use

Specifications in detail

• Input: AHL 5-50nM
• Output: Visible fluorescent protein
• Response Time: < 3 hours
• Operating conditions
• Health & safety
• Packaging and shelf-life

References