From 2007.igem.org
Wet Lab: Protocols
Below is a databank of all the protocols that have been used in our projects.
| General Experiments | Testing Experiments
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Protein Purification
- Purification of LuxR : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]
- Purification of GFPmut3b: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.6 Results]
Fluorometer Testing
- Optimisation of Fluorometer: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.1 Results]
Calibration Curve
- Calibration Curve for GFPmut3b: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.3 Results]
- Degradation Curve for GFPmut3b :Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.4 Results]
Chassis Protocols
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pT7 Characterisation
- pT7 in vitro: Protocols and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.9 Results]
Cell by Date
Implementation
- In Vivo Testing: Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results]
- Initial In vitro Testing:Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results]
Testing/Validation
- Testing DNA Concentration in vitro: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD4 |Results]
- Operating Temperature Range :Protocol 1 and Protocol 2 and Results
- Temperature Steps: Protocols and Results
Infecter Detector
Implementation
Testing/Validation
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