Imperial/Cell-Free/Applications
From 2007.igem.org
Cell-Free: Applications
Our Design
To get around the problem of having bacteria in contact with food or medical devices in the applications of Infector Detector and Cell by Date respectively, we aimed to express reporter DNA constructs within a CFS consisting of various E. coli cell extracts (homemade S30, commercial S30 and commercial S30 pT7) in bulk solution. In parallel, we investigated and attempted to optimize conditions for phospholipid vesicle formation. Future work on our project would then involve combining the two, so that protein expression is achieved inside vesicles that have prolonged chassis lifespan.
(Details of protocols to be inserted here).
Cell-Free in Infector Detector
For more details on how Infector Detector integrates CFS into its system, go to its Design page.
Cell-Free in Cell by Date
For more details on how Cell by Date utlizes CFS, go to its Design page. For more details on the determination of CFS capabilities and characterizing it, go to its Testing/Validation page.
