Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments

Testing Experiments

Protein Purification

Purification of LuxR: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]
Purification of GFPmut3b: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.6 Results]

Fluorometer Testing

Optimisation of Fluorometer: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.1 Results]

Calibration Curve

Calibration Curve for GFPmut3b: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.3 Results]
Degradation Curve for GFPmut3b:Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.4 Results]

Chassis Protocols

pT7 Characterisation

pT7 in vitro: [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/Protocols/Prot1.9 Protocols] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.9 Results]

Cell by Date
Implementation

In Vivo Testing: Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results]
Initial In vitro Testing: Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results]

Testing/Validation

Testing DNA Concentration in vitro: Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD4 Results]
Operating Temperature Range: Protocol 1 and Protocol 2 and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.2 Results 1] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
Temperature Steps: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]

Infecter Detector
Implementation

In vivo Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]
Initial In Vitro Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]

Testing/Validation

Testing of DNA concentration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res2.1 Results]
Testing of Purified LuxR: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]
Characterization in vitro - AHL titration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 Results]

Imperial/Wet Lab/Protocols