Imperial/Cell by Date/Implementation
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Revision as of 13:25, 24 October 2007 by Alexander.wong (Talk | contribs)
Cell by Date: Implementation
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| Construct Design | 
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| Stage 1: Availability | Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry | Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry. | |||||
| Stage 2: Cloning / Amplification |  |  | |||||
| Stage 3: Ligation | Not Required | Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
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| Stage 4: Purification of Activator Proteins | Not Required | Not Required | |||||
| Stage 5: Quality Control |
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| Stage 6: Final Implementation Status | Ready for Testing | Ready for Testing | |||||
DNA Construct Status
| Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry |
| Notes: The part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] |
Constitutive tetR Promoter
Ligation Strategy:
N/A
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at the testing page. |
| Sequencing | Pending | Sequence results not obtained yet |
Constitutive T7 Promoter
Ligation Strategy:
| + | 
| = |
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Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at ----- |
| Sequencing | Pending | Sequence results not obtained yet |
Chassis Status
cell extract preparation protocols.
