Imperial/Wet Lab/Protocols

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Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments

Testing Experiments

Protein Purification

Purification of LuxR: Protocol and Results
Purification of GFPmut3b: Protocol and Results

Fluorometer Testing

Optimisation of Fluorometer: Protocol and Results

Calibration Curve

Calibration Curve for GFPmut3b: Protocol and Results
Degradation Curve for GFPmut3b:Protocol and Results

Chassis Protocols

Reaction Mixture of Commercially available S30 Cell Extract :Protocol
Reaction Mixture of Homemade S30 Cell Extract :Protocol
Recipe for S30 Cell Extract :Protocol
Recipe for S12 Cell Extract :Protocol
Testing of Homemade S30 Cell Extract with ID construct :Protocol and Results
Vesicles Formation: Protocol

pT7 Characterisation

pT7 in vitro: Protocol and Results

Cell by Date
Implementation

In Vivo Testing: Protocols and Results
Initial In vitro Testing: Protocols and Results

Testing/Validation

Testing DNA Concentration in vitro: Protocols and Results
Isothermal experiments: Protocols and Results
Temperature Steps: Protocols and Results
Packaging experments: Protocols and Results

Infector Detector
Implementation

In vivo Testing: Protocol and Results
Initial In Vitro Testing: Protocol and Results

Testing/Validation

Testing of DNA concentration : Protocol and Results
Testing of Purified LuxR: Protocol and Results
Characterization in vitro - AHL titration : Protocol and Results

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