Imperial

From 2007.igem.org

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                           <td>James Chappell</td>
                           <td>James Chappell</td>
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                           <td style="text-align: right; padding-left: 5px;">Prof Paul Freemont</td>
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                           <td style="text-align: right; padding-left: 5px;">Prof. Paul Freemont</td>
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                           <td>Lucas Hadjilucas</td>
                           <td>Lucas Hadjilucas</td>
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                           <td style="text-align: right; padding-left: 5px;">Prof Richard Kitney</td>
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                           <td style="text-align: right; padding-left: 5px;">Prof. Richard Kitney</td>
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Revision as of 00:47, 20 October 2007

Project Overview

Our contribution to the synthetic biology community this year will be the investigation of Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built.

Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

Main Achievements

  • Designed and modelled a device to detect biofilms and potentially prevent diseases like MRSA
  • Characterised Infector Detector in terms of various AHL concentrations, at room temperature

Engineering Development Cycle



 Image for Specs.
SPECIFICATIONS
Image for Testing/Validation.
TESTING / VALIDATION 		Image for full system.
 Image for Design.
DESIGN

Image for full system.
INFECTOR DETECTOR
Image for Implementation.
IMPLEMENTATION
Image for Modelling.
MODELLING

Foundational technology - Cell-Free Systems

Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification requirements. Click here for more details!

Main Achievements

  • Investigated and characterised the in vitro chassis
  • Characterised the F2620, pTet-GFP and pT7-GFP construct in vitro

Sub Project - Cell by Date

Cell by Date will report when beef has been out of the fridge for too long. It produces fluorescent proteins at ambient temperatures above 8°C. (more...)

Main Achievements

  • Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system

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