Imperial/Cell-Free/Contribution
From 2007.igem.org
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== ''In vitro'' Expression == | == ''In vitro'' Expression == | ||
| - | + | *Attempted to make S30 ''E. coli'' cell extract and feeding solution | |
| - | + | *Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP | |
| + | *Successfully used homemade S30 ''E. coli'' cell extract with commercial feeding solution for expression of GFP | ||
| + | *Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 ''E. coli'' cell extract and feeding solution | ||
== Vesicles Formation == | == Vesicles Formation == | ||
| - | + | *Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer | |
| - | + | *Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract | |
| + | *Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs | ||
| + | *Attempting to find suitable pore proteins to prolong vesicle and expression lifespan | ||
== Documentation on Registry of Parts == | == Documentation on Registry of Parts == | ||
| + | Documented our findings with the following Registry Additions: | ||
| + | * 1 | ||
| + | * 2 | ||
| + | * 3 | ||
Revision as of 11:15, 19 October 2007
In vitro Expression
- Attempted to make S30 E. coli cell extract and feeding solution
- Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
- Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
- Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution
Vesicles Formation
- Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
- Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
- Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
- Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
Documentation on Registry of Parts
Documented our findings with the following Registry Additions:
- 1
- 2
- 3
