Imperial/Cell-Free/Contribution
From 2007.igem.org
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| - | == Our Contribution == | + | == Cell-Free: Our Contribution == |
=== ''In vitro'' Expression === | === ''In vitro'' Expression === | ||
*Attempted to make S30 ''E. coli'' cell extract and feeding solution | *Attempted to make S30 ''E. coli'' cell extract and feeding solution | ||
Revision as of 22:20, 20 October 2007
Cell-Free: Our Contribution
In vitro Expression
- Attempted to make S30 E. coli cell extract and feeding solution
- Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
- Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
- Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution
Vesicles Formation
- Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
- Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
- Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
- Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
Documentation on Registry of Parts
Documented our findings with the following Registry Additions:
- 1
- 2
- 3
