Imperial/Cell-Free/Contribution

From 2007.igem.org

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(Documentation on Registry of Parts)
(Documentation on Registry of Parts)
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=== Documentation on Registry of Parts ===
=== Documentation on Registry of Parts ===
Documented our findings with the following Registry Additions:
Documented our findings with the following Registry Additions:
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* [http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]
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[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]
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** [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade ''E. coli'' S30]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade ''E. coli'' S30]
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** [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial ''E. coli'' S30]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial ''E. coli'' S30]
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** [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial ''E. coli'' T7 S30]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial ''E. coli'' T7 S30]
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** [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]
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Revision as of 01:26, 22 October 2007


Cell-Free: Our Contribution

In vitro Expression

  • Attempted to make S30 E. coli cell extract and feeding solution
  • Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
  • Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
  • Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution


Vesicles Formation

  • Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
  • Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
  • Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
  • Attempting to find suitable pore proteins to prolong vesicle and expression lifespan


Documentation on Registry of Parts

Documented our findings with the following Registry Additions:

[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade E. coli S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial E. coli S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial E. coli T7 S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]



<< Cell-Free vs. Cell | Our Contribution | Characterisation >>