Imperial/Cell-Free/Contribution

From 2007.igem.org

< Imperial(Difference between revisions)
(Documentation on Registry of Parts)
 
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== Cell-Free: Our Contribution ==
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= Cell-Free: Our Contribution =
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=== ''In vitro'' Expression ===
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== ''In vitro'' Expression ==
*Attempted to make S30 ''E. coli'' cell extract and feeding solution
*Attempted to make S30 ''E. coli'' cell extract and feeding solution
*Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
*Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
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=== Vesicles Formation ===
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== Vesicles Formation ==
*Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
*Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
*Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract
*Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract
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=== Documentation on Registry of Parts ===
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== Documentation on Registry of Parts ==
Documented our findings with the following Registry Additions:
Documented our findings with the following Registry Additions:

Latest revision as of 19:55, 26 October 2007



Cell-Free: Our Contribution

In vitro Expression

  • Attempted to make S30 E. coli cell extract and feeding solution
  • Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
  • Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
  • Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution


Vesicles Formation

  • Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
  • Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
  • Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
  • Attempting to find suitable pore proteins to prolong vesicle and expression lifespan


Documentation on Registry of Parts

Documented our findings with the following Registry Additions:

[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]

  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade E. coli S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial E. coli S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial E. coli T7 S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]



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