From 2007.igem.org

Revision as of 13:10, 20 October 2007 (view source) Alexander.wong (Talk | contribs) (→Constructs) ← Older edit		Revision as of 17:06, 21 October 2007 (view source) Ben Yi (Talk | contribs) Newer edit →
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	{{Template:IC07navmenu}}		{{Template:IC07navmenu}}
	__NOTOC__		__NOTOC__
		+	{|border=1 width=90% align="center"
		+	|-
		+	|width="30%"|<center>[[Image:Imperial CBD Design CBD1.png ]]</center>
		+	|width="--"|Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
		+	|-
		+	|<center>[[Image:Imperial CBD Design CBD2.png]]</center>
		+	|width="--"|Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
		+	|}
		+
		+	===Construct Status===
		+	=====Constitutive tetracyclin promoter=====
		+	<center>[[Image:Imperial CBD Design CBD1.png]]</center>
		+	'''Quality Control:'''
		+	{|border=1 width=90% align="center"
		+	|-
		+	|width="20%"|&nbsp;Testing ''in vivo''
		+	|width="10%" align="center"|Done
		+	|width="--"|Results can be found at -----
		+	|-
		+	|&nbsp;Testing ''in vitro''
		+	|align="center"|Done
		+	|Results can be found at the [[Imperial/Cell_by_Date/Testing|testing]] page.
		+	|-
		+
		+	|&nbsp;Sequencing
		+	|align="center"|Pending
		+	|''Sequence results not obtained yet''
		+	|}
		+
		+
		+	=====Constitutive T7 promoter=====
		+	<center>[[Image:Imperial CBD Design CBD2.png]]</center>
		+
		+	'''Ligation Strategy:'''
		+	{|
		+	|-
		+	|T7
		+	|<font size="+3">+</font>
		+	|GFP
		+	|<font size="+3">=</font>
		+	|[[Image:Imperial CBD Design CBD2.png]]
		+	|}
		+
		+	'''Quality Control:'''
		+	{|border=1 width=90% align="center"
		+	|-
		+	|width="20%"|&nbsp;Testing ''in vivo''
		+	|width="10%" align="center"|Done
		+	|width="--"|Results can be found at -----
		+	|-
		+	|&nbsp;Testing ''in vitro''
		+	|align="center"|Done
		+	|Results can be found at -----
		+	|-
		+
		+	|&nbsp;Sequencing
		+	|align="center"|Pending
		+	|''Sequence results not obtained yet''
		+	|}
-	== Constructs ==
-	(Ligation Strategy??)
-	(Quality Control??)
-	Sequencing of Parts?
	== Chassis ==		== Chassis ==
	cell extract preparation protocols.		cell extract preparation protocols.

---

Revision as of 17:06, 21 October 2007

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

	Notes: This part can be found as BBa_I13522 in the registry
	Notes: the part BBa_E7104 we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick BBa_I13504

Construct Status

Constitutive tetracyclin promoter

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at the testing page.
Sequencing	Pending	Sequence results not obtained yet

Constitutive T7 promoter

Ligation Strategy:

T7

+

GFP

=

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at -----
Sequencing	Pending	Sequence results not obtained yet

Chassis

cell extract preparation protocols.