Imperial/Cell by Date/Implementation
From 2007.igem.org
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|<center>[[Image:Imperial CBD Design CBD2.png]]</center> | |<center>[[Image:Imperial CBD Design CBD2.png]]</center> | ||
| - | |width="--"|Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not | + | |width="--"|Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] |
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Revision as of 20:54, 21 October 2007
DNA Constructs
 | Notes: This part can be found as BBa_I13522 in the registry |
 | Notes: the part BBa_E7104 we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick BBa_I13504 |
Constitutive tetR Promoter
Ligation Strategy:
N/A
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at the testing page. |
| Sequencing | Pending | Sequence results not obtained yet |
Constitutive T7 Promoter
Ligation Strategy:
| + | 
| = |
|
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at ----- |
| Sequencing | Pending | Sequence results not obtained yet |
Chassis
cell extract preparation protocols.
