From 2007.igem.org

Revision as of 01:59, 24 October 2007 (view source) Alexander.wong (Talk | contribs) ← Older edit		Revision as of 12:34, 24 October 2007 (view source) Alexander.wong (Talk | contribs) Newer edit →
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	__NOTOC__		__NOTOC__
	= Cell by Date: Implementation =		= Cell by Date: Implementation =
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		+	{|border=1 width=100% align="center" cellpadding="10px"
		+	|-
		+	|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center>
		+	|- align="center"
		+	|wifth="20%" style="background-color:#ddeeff"| '''Construct Design'''
		+	|width="40%"| [[Image:Imperial CBD Design CBD1.png]]
		+	|width="40%"| [[Image:Imperial CBD Design CBD2.png]]
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
		+	|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry
		+	|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
		+	|width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]]
		+	|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]]
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
		+	|width="40%"| Not Required
		+	|width="40%"| Not Required
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
		+	|width="40%"| Not Required
		+	|width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results])
		+	|-
		+	|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
		+	|width="40%"|
		+	* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results])
		+	* '''Sequencing of Construct''': Pending
		+	|width="40%"|
		+	* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results])
		+	* '''Sequencing of Construct''': Pending
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
		+	|width="40%" style="background-color:#b8efb0"| Ready for Testing
		+	|width="40%" style="background-color:#ddb99e"| Aborted. <br /> LuxR protein not functional.
		+	|}
		+	<br clear="all">
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	== DNA Construct Status ==		== DNA Construct Status ==

---

Revision as of 12:34, 24 October 2007

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

---

Cell by Date: Implementation

DNA Construct Implementation
Construct Design
Stage 1: Availability	Found as BBa_I13522 in Registry	Found as BBa_E7104 in Registry.
Stage 2: Cloning / Amplification	BBa_T9002 - Lane 18 (1945bp)	BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation	Not Required	Not Required
Stage 4: Purification of Activator Proteins	Not Required	LuxR protein (Protocol and Results)
Stage 5: Quality Control	Testing in vivo: Done (Protocol and Results) Sequencing of Construct: Pending	Testing in vitro: Done (Protocol and Results) Sequencing of Construct: Pending
Stage 6: Final Implementation Status	Ready for Testing	Aborted. LuxR protein not functional.

DNA Construct Status

	Notes: This part can be found as BBa_I13522 in the registry
	Notes: The part BBa_E7104 we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick BBa_I13504

Constitutive tetR Promoter

Ligation Strategy:

N/A

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at the testing page.
Sequencing	Pending	Sequence results not obtained yet

Constitutive T7 Promoter

Ligation Strategy:

+

=

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at -----
Sequencing	Pending	Sequence results not obtained yet

Chassis Status

cell extract preparation protocols.

Navigation

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