Imperial/Cell by Date/Implementation
From 2007.igem.org
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|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' | ||
| - | |width="40%"| [[Image: | + | |width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]] |
| - | |width="40%"| [[Image: | + | |width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' | |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' | ||
|width="40%"| Not Required | |width="40%"| Not Required | ||
| - | |width="40%"| | + | |width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] |
| + | <br> | ||
| + | {| width="80%" | ||
| + | |- | ||
| + | |[[Image:Imperial_CBD_Design_CBD4.png|30px]] | ||
| + | |<font size="+3">+</font> | ||
| + | |[[Image:Imperial_CBD_Design_CBD3.png|110px]] | ||
| + | |<font size="+3">=</font> | ||
| + | |[[Image:Imperial CBD Design CBD2.png|130px]] | ||
| + | |} | ||
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' | ||
|width="40%"| Not Required | |width="40%"| Not Required | ||
| - | |width="40%"| | + | |width="40%"| Not Required |
|- | |- | ||
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | ||
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* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in | + | * '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]) |
* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | ||
|width="40%" style="background-color:#b8efb0"| Ready for Testing | |width="40%" style="background-color:#b8efb0"| Ready for Testing | ||
| - | |width="40%" style="background-color:# | + | |width="40%" style="background-color:#b8efb0"| Ready for Testing |
|} | |} | ||
<br clear="all"> | <br clear="all"> | ||
Revision as of 13:25, 24 October 2007
Cell by Date: Implementation
| | |||||||
| Construct Design | 
| 
| |||||
| Stage 1: Availability | Found as BBa_I13522 in Registry | Found as BBa_E7104 in Registry. | |||||
| Stage 2: Cloning / Amplification |  |  | |||||
| Stage 3: Ligation | Not Required | Since BBa_E7104 was not functional, we made our own promoter and and ligated it to BBa_I13504
| |||||
| Stage 4: Purification of Activator Proteins | Not Required | Not Required | |||||
| Stage 5: Quality Control | |||||||
| Stage 6: Final Implementation Status | Ready for Testing | Ready for Testing | |||||
DNA Construct Status
| Notes: This part can be found as BBa_I13522 in the registry |
| Notes: The part BBa_E7104 we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick BBa_I13504 |
Constitutive tetR Promoter
Ligation Strategy:
N/A
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at the testing page. |
| Sequencing | Pending | Sequence results not obtained yet |
Constitutive T7 Promoter
Ligation Strategy:
| + | 
| = |
|
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at ----- |
| Sequencing | Pending | Sequence results not obtained yet |
Chassis Status
cell extract preparation protocols.
