Imperial/Cell by Date/Implementation

From 2007.igem.org

(Difference between revisions)
(→Cell by Date: Implementation)
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|width="40%"| '''Concept 2'''<br />[[Image:Imperial CBD Design CBD2.1.png|center]]
|width="40%"| '''Concept 2'''<br />[[Image:Imperial CBD Design CBD2.1.png|center]]
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry
|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry
|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]]
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]]
|width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]]
|width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]]
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
|width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
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|}
|}
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Not Required
|-  
|-  
-
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
+
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
+
* '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
* '''Sequencing of Construct''': Pending
* '''Sequencing of Construct''': Pending
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
+
* '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
* '''Sequencing of Construct''': Pending
* '''Sequencing of Construct''': Pending
|- align="center"
|- align="center"
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|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Possibility of making Homemade S30 Cell Extract.
|width="40%"| Possibility of making Homemade S30 Cell Extract.
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
|-  
|-  
-
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control'''
+
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vitro''''': Done (Protocol and Results)
+
* '''Testing ''in vitro''''': Passed (Protocol and Results)
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results])
+
* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results])
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status'''
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
|width="40%" style="background-color:#b8efb0"| Ready for Testing
|width="40%" style="background-color:#b8efb0"| Ready for Testing

Revision as of 14:30, 24 October 2007


Cell by Date: Implementation

DNA Construct Implementation
Construct Design Concept 1
Imperial CBD Design CBD1.png
 Concept 2
Imperial CBD Design CBD2.1.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png 		Found as BBa_I13522 in Registry Found as BBa_E7104 in Registry.
Stage 2: Cloning / Amplification

Icegems-Devcycle-downarrow.png
BBa_I13522 - Lane R2 (937bp + 2079bp vector)
BBa_E7104 - Lane 11 (Missing 826bp)
Stage 3: Ligation

Icegems-Devcycle-downarrow.png 		Not Required Since BBa_E7104 was not functional, we made our own promoter and and ligated it to BBa_I13504


Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.2.png
Stage 4: Purification of Activator Proteins

Icegems-Devcycle-downarrow.png 		Not Required Not Required
Stage 5: Quality Control

Icegems-Devcycle-downarrow.png
  • Testing in vivo: Passed (Protocols and Results)
  • Sequencing of Construct: Pending
  • Testing in vivo: Passed (Protocols and Results)
  • Sequencing of Construct: Pending
Stage 6: Final Implementation Status Ready for Testing Ready for Testing



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png 		Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation

Icegems-Devcycle-downarrow.png 		Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control

Icegems-Devcycle-downarrow.png
  • Testing in vitro: Passed (Protocol and Results)
Stage 4: Final Implementation Status Aborted.
Expression capacity of chassis was low. 		Ready for Testing



Navigation

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