Revision as of 03:07, 26 October 2007

Cell by Date: Implementation

DNA Construct Implementation

Construct Design

Concept 1

Concept 2

Stage 1: Availability

Found as BBa_I13522 in Registry

Found as BBa_E7104 in Registry.

Stage 2: Cloning / Amplification

BBa_I13522 - Lane R2 (937bp + 2079bp vector)

BBa_E7104 - Lane 11 (Missing 826bp)

Stage 3: Ligation

Not Required

Since BBa_E7104 was not functional, we made our own promoter and and ligated it to BBa_I13504

+

=

Stage 4: Purification of Activator Proteins

Not Required

Stage 5: Quality Control

Stage 6: Final Implementation Status

Ready for Testing

Chassis Construct Implementation
Chassis Design
Stage 1: Availability	Possibility of making Homemade S30 Cell Extract.	Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation	Preparation: Done (Protocol)	Preparation: Done (Protocol)
Stage 3: Quality Control	Testing in vitro: Passed (Protocol and Results)	Testing in vitro: Passed (Protocols and Results)
Stage 4: Final Implementation Status	Aborted. Expression capacity of chassis was low.	Ready for Testing

<< Modelling | Implementation | Testing/Validation >>

@@ Line 26: / Line 26: @@
 |- align="left"
 |wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design'''
-|width="40%"| '''Concept 1'''<br />[[Image:Imperial CBD Design CBD1.png|center]]
+|width="40%"| '''Concept 1'''<br />[[Image:IC07_design_CBDcon1.png|center|370px]]
-|width="40%"| '''Concept 2'''<br />[[Image:Imperial CBD Design CBD2.1.png|center]]
+|width="40%"| '''Concept 2'''<br />[[Image:IC07_design_CBDcon2.png|center|370px]]
 |- align="center"
 |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]