Imperial/Cell by Date/Implementation
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{{Template:IC07navmenu}} | {{Template:IC07navmenu}} | ||
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<html> | <html> | ||
<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /> | <link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /> | ||
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|- align="left" | |- align="left" | ||
|wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design''' | |wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design''' | ||
| - | |width="40%"| '''Concept 1'''<br />[[Image: | + | |width="40%"| '''Concept 1'''<br />[[Image:IC07_design_CBDcon1.png|center|370px]] |
| - | |width="40%"| '''Concept 2'''<br />[[Image: | + | |width="40%"| '''Concept 2'''<br />[[Image:IC07_design_CBDcon2.png|center|370px]] |
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry | |width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry | ||
|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry. | |width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry. | ||
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]] | |width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]] | ||
|width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]] | |width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]] | ||
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| Not Required | |width="40%"| Not Required | ||
|width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] | |width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] | ||
| Line 43: | Line 44: | ||
{| width="80%" | {| width="80%" | ||
|- | |- | ||
| - | |[[Image:Imperial_CBD_Design_CBD4.png| | + | |[[Image:Imperial_CBD_Design_CBD4.png|37px]] |
|<font size="+3">+</font> | |<font size="+3">+</font> | ||
|[[Image:Imperial_CBD_Design_CBD3.png|110px]] | |[[Image:Imperial_CBD_Design_CBD3.png|110px]] | ||
| Line 50: | Line 51: | ||
|} | |} | ||
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| Not Required | |width="40%"| Not Required | ||
|width="40%"| Not Required | |width="40%"| Not Required | ||
|- | |- | ||
| - | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | + | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
| - | |width="40%"| | + | |width="40%"| [[Image:IC2007_Experiments_Phase1_Protocol1-1.jpg|center|350px]]<br /> |
| - | * '''Testing ''in vivo''''': | + | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.1 Results]) |
| - | * '''Sequencing of Construct''': | + | * '''Sequencing of Construct''': Completed |
| - | |width="40%"| | + | |width="40%"| [[Image:IC_2007_PT7-invitro.png|center|350px]]<br /> |
| - | * '''Testing ''in | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.9|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 Results]) |
| - | * '''Sequencing of Construct''': | + | * '''Sequencing of Construct''': Completed |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | ||
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|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]] | |colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]] | ||
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| Possibility of making Homemade S30 Cell Extract. | |width="40%"| Possibility of making Homemade S30 Cell Extract. | ||
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. | |width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. | ||
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]) | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]) | ||
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | ||
|- | |- | ||
| - | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' | + | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]]) |
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.2 Results]) |
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage | + | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' |
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | |width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | ||
|width="40%" style="background-color:#b8efb0"| Ready for Testing | |width="40%" style="background-color:#b8efb0"| Ready for Testing | ||
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| - | + | ||
<center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center> | <center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center> | ||
Latest revision as of 02:35, 27 October 2007
Cell by Date: Implementation
| | |||||||
| Construct Design | Concept 1 | Concept 2 | |||||
Stage 1: Availability 
| Found as BBa_I13522 in Registry | Found as BBa_E7104 in Registry. | |||||
| Stage 2: Cloning / Amplification
|  |  | |||||
| Stage 3: Ligation
| Not Required | Since BBa_E7104 was not functional, we made our own promoter and and ligated it to BBa_I13504
| |||||
| Stage 4: Purification of Activator Proteins
| Not Required | Not Required | |||||
| Stage 5: Quality Control
|  |  | |||||
| Stage 6: Final Implementation Status | Ready for Testing | Ready for Testing | |||||
| | ||
| Chassis Design | 
| |
| Stage 1: Availability
| Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from Promega. |
| Stage 2: Preparation
| Preparation: Done (Protocol) | Preparation: Done (Protocol) |
| Stage 3: Quality Control
| ||
| Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |
