Imperial/Cell by Date/Implementation

From 2007.igem.org

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(Constitutive tetracyclin promoter)
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===Constitutive tetracyclin promoter===
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===Constitutive tetR Promoter===
<center>[[Image:Imperial CBD Design CBD1.png]]</center>
<center>[[Image:Imperial CBD Design CBD1.png]]</center>
'''Ligation Strategy:'''
'''Ligation Strategy:'''
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|''Sequence results not obtained yet''
|''Sequence results not obtained yet''
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===Constitutive T7 promoter===
===Constitutive T7 promoter===

Revision as of 20:50, 21 October 2007


DNA Constructs

Imperial CBD Design CBD1.png
Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
Imperial CBD Design CBD2.png
Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Constitutive tetR Promoter

Imperial CBD Design CBD1.png

Ligation Strategy:

N/A

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at the testing page.
 Sequencing Pending Sequence results not obtained yet

Constitutive T7 promoter

Imperial CBD Design CBD2.png

Ligation Strategy:

Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.png

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at -----
 Sequencing Pending Sequence results not obtained yet



Chassis

cell extract preparation protocols.