Imperial/Cell by Date/Implementation

From 2007.igem.org

(Difference between revisions)
(Cell by Date: Implementation)
(Cell by Date: Implementation)
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|wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design'''
|wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design'''
|width="40%"| '''Concept 1'''<br />[[Image:Imperial CBD Design CBD1.png|center]]
|width="40%"| '''Concept 1'''<br />[[Image:Imperial CBD Design CBD1.png|center]]
-
|width="40%"| '''Concept 2'''<br />[[Image:Imperial CBD Design CBD2.png|center]]
+
|width="40%"| '''Concept 2'''<br />[[Image:Imperial CBD Design CBD2.1.png|center]]
|- align="center"
|- align="center"
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''

Revision as of 14:08, 24 October 2007



Cell by Date: Implementation

DNA Construct Implementation
Construct Design Concept 1
Imperial CBD Design CBD1.png
Concept 2
Imperial CBD Design CBD2.1.png
Stage 1: Availability Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
Stage 2: Cloning / Amplification
BBa_I13522 - Lane R2 (937bp + 2079bp vector)
BBa_E7104 - Lane 11 (Missing 826bp)
Stage 3: Ligation Not Required Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.png
Stage 4: Purification of Activator Proteins Not Required Not Required
Stage 5: Quality Control
  • Testing in vivo: Done (Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
  • Sequencing of Construct: Pending
  • Testing in vivo: Done (Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
  • Sequencing of Construct: Pending
Stage 6: Final Implementation Status Ready for Testing Ready for Testing



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control
  • Testing in vitro: Done (Protocol and Results)
  • Testing in vitro: Done (Protocols and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results])
Stage 6: Final Implementation Status Aborted.
Expression capacity of chassis was low.
Ready for Testing



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