From 2007.igem.org

Revision as of 17:06, 21 October 2007 (view source) Ben Yi (Talk | contribs) ← Older edit		Revision as of 17:07, 21 October 2007 (view source) Ben Yi (Talk | contribs) m Newer edit →
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		+	== Constructs ==
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Revision as of 17:07, 21 October 2007

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Constructs

	Notes: This part can be found as BBa_I13522 in the registry
	Notes: the part BBa_E7104 we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick BBa_I13504

Construct Status

Constitutive tetracyclin promoter

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at the testing page.
Sequencing	Pending	Sequence results not obtained yet

Constitutive T7 promoter

Ligation Strategy:

T7

+

GFP

=

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at -----
Sequencing	Pending	Sequence results not obtained yet

Chassis

cell extract preparation protocols.