Imperial/Cell by Date/Implementation
From 2007.igem.org
(Difference between revisions)
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|width="--"|Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] | |width="--"|Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] | ||
|} | |} | ||
| + | <br clear="all"> | ||
| - | + | ===Constitutive tetracyclin promoter=== | |
| - | + | ||
<center>[[Image:Imperial CBD Design CBD1.png]]</center> | <center>[[Image:Imperial CBD Design CBD1.png]]</center> | ||
| + | '''Ligation Strategy:''' | ||
| + | |||
| + | N/A | ||
| + | |||
'''Quality Control:''' | '''Quality Control:''' | ||
{|border=1 width=90% align="center" | {|border=1 width=90% align="center" | ||
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| - | + | ===Constitutive T7 promoter=== | |
<center>[[Image:Imperial CBD Design CBD2.png]]</center> | <center>[[Image:Imperial CBD Design CBD2.png]]</center> | ||
Revision as of 20:47, 21 October 2007
DNA Constructs
 | Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry |
 | Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] |
Constitutive tetracyclin promoter
Ligation Strategy:
N/A
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at the testing page. |
| Sequencing | Pending | Sequence results not obtained yet |
Constitutive T7 promoter
Ligation Strategy:
| + | 
| = |
|
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at ----- |
| Sequencing | Pending | Sequence results not obtained yet |
Chassis
cell extract preparation protocols.
