Imperial/Cell by Date/Implementation
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Revision as of 12:34, 24 October 2007 by Alexander.wong (Talk | contribs)
Cell by Date: Implementation
| ||
Construct Design | ||
Stage 1: Availability | Found as BBa_I13522 in Registry | Found as BBa_E7104 in Registry. |
Stage 2: Cloning / Amplification | ||
Stage 3: Ligation | Not Required | Not Required |
Stage 4: Purification of Activator Proteins | Not Required | LuxR protein (Protocol and Results) |
Stage 5: Quality Control | ||
Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
DNA Construct Status
Notes: This part can be found as BBa_I13522 in the registry | |
Notes: The part BBa_E7104 we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick BBa_I13504 |
Constitutive tetR Promoter
Ligation Strategy:
N/A
Quality Control:
Testing in vivo | Done | Results can be found at ----- |
Testing in vitro | Done | Results can be found at the testing page. |
Sequencing | Pending | Sequence results not obtained yet |
Constitutive T7 Promoter
Ligation Strategy:
+ | = |
Quality Control:
Testing in vivo | Done | Results can be found at ----- |
Testing in vitro | Done | Results can be found at ----- |
Sequencing | Pending | Sequence results not obtained yet |
Chassis Status
cell extract preparation protocols.