Imperial/Dry Lab/Modelling

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(Selection of Model Design and Structure)
 
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=Model Development for Infector Detector=
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==Formulation of the problem==
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<center>'''Welcome to the Modelling Sub-Portal Page'''</center><br><br>
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As described earlier, catheter-associated urinary tract infection (CAUTI) in the clinical setting is a prevalent problem with extensive economic impact. The underlying cause of many such infections can be attributed to the formation of biofilm, by aggregating-bacteria on the surface of urinary catheters.
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<center>This page serves as a shuttle to the modelling phase of each project:Infector Detector and Cell-by-Date. </center>
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[[Image: IC07_QS.png|right|thumb|500px| Role of AHL (HSL) quorum-sensing in biofilm formation]]
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<center>Select one of the following links to be transferred to the modelling of the relevant project.</center>
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Infector Detector (ID) is a simple biological detector, which serves to expose bacterial biofilm. It functions by exploiting the inherent AHL (Acetyl Homoserine Lactone)  production employed by certain types of quorum-sensing bacteria, in the formation of such structures.<br>
 
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Our project attempts to improve where previous methods of biofilm detection have proven ineffective: first and foremost, by focussing on the sensitivity of the system, to markers of biofilm: in this case, low levels of AHL production (which represents the bacterial "chatter" of such aggregating bacteria).
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{{Click || image = ID ModelPage.jpg| link = Imperial/Infector_Detector/Modelling | width = 200px | height = 198px }}
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<br> [[Imperial/Infector_Detector/Modelling| '''Infector Detector''']]
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{{Click || image = ThermoDA.jpg| link = Imperial/Cell_by_Date/Modelling | width = 200px | height = 200px }}<br>
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[[Imperial/Cell_by_Date/Modelling| '''Cell-by-Date''']]
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In doing so, a complete investigation of the level of sensitivity to AHL concentration needs to be performed - in other words, what is the minimal AHL concentration for appreciable expression of a chosen reporter protein. Furthermore, establish a functional range for possible AHL detection. How does increased AHL concentration impact on the maximal output of reporter protein?<br>
 
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Finally, how can the system performance be tailored, by exploiting possible state variables (e.g. varying initial LuxR concentration and/or concentration of pLux promoters). 
 
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The system performance here revolves most importantly around AHL sensitivity; however, the effect on the maximal output of fluorescent reporter protein and response time is, likewise, of great importance.
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<center> | [[Imperial/Dry_Lab | Dry Lab >>]]</center>
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==Selection of Model Design and Structure==
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Since the novelty of our solution revolves around the use of cell-free systems as a baterial-free solution in the clinical setting, a simple system is selected. This solution, involves the proposal of two simple constructs, varying with respect to the manner in which LuxR is introduced into the system:
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*Construct 1 - represented by [http://partsregistry.org/Part:BBa_T9002| T9002], incorporates constitutive expression of LuxR by pTET.
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*Construct 2 - simpler in nature, lacks pTET; LuxR is introduced in purified form here.<br>
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<font color = red>~~Here explain briefly why Construct 2 was selected, i.e. we were concerned with the time the system would take to reach steady-state (that is before energy-dependence was considered) - due to almost negligible <math> \delta_{LuxR}</math>, etc </font>.
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==References==
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Latest revision as of 02:10, 27 October 2007



Welcome to the Modelling Sub-Portal Page


This page serves as a shuttle to the modelling phase of each project:Infector Detector and Cell-by-Date.

Select one of the following links to be transferred to the modelling of the relevant project.



Infector Detector


Cell-by-Date



| Dry Lab >>