Imperial/Infector Detector/Modelling

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  • Introduction
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  • Implementation
  • Testing/Validation
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Introduction

Infector Detector (ID) is a simple biological detector, which serves to expose bacterial biofilm. It functions by exploiting the inherent AHL (Acetyl Homoserine Lactone) production employed by certain types of quorum-sensing bacteria, in the formation of such structures.

In doing so, a complete investigation of the level of sensitivity to AHL concentration needs to be performed -in other words, what is the minimal AHL concentration for appreciable expression of a chosen reporter protein. Furthermore, establish a functional range for possible AHL detection. How does increased AHL concentration impact on the maximal output of reporter protein?
Finally, how can the system performance be tailored, by exploiting possible state variables (e.g. varying initial LuxR concentration and/or concentration of pLux promoters).

The system performance here revolves most importantly around AHL sensitivity; however, the effect on the maximal output of fluorescent reporter protein and response time is, likewise, of great importance.

Implementation & Reaction Network

Construct 1

Construct 2

Representative Model

Model 2, an energy-dependent network, where the dependence on energy assumes Hill-like dynamics

Simulations

Sensitivity Analysis?

Conclusions