Imperial/Wet Lab/DNA Constructs
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Latest revision as of 02:16, 27 October 2007
Wet Lab: DNA Constructs
Constructs Overview
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details.
Cloning Table
Status of biobricks cloned and their applications:
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low]
I | ID | Part | Description | Status | Ligation Target | Use in Constructs |
I | 02 | BBa_I13522 | ptet-GFP | XL1, Maxi, Mini, BL21 | --- | Cell By Date concept 1 |
11 | BBa_E7104 | pT7 GFP | XL1, Maxi, Midi, Mini, BL21 | --- | Cell By Date concept 2 | |
30 | --- | pT7 (strong GFP) | XL1, Maxi, Mini, BL21 | --- | Construct to test T7 promoter | |
I | 18 | BBa_T9002 | ptet-luxR-plux-GFP | XL1, Maxi, Midi, BL21 | --- | Infector Detector construct 1 |
05 | BBa_R0040 | ptet | XL1, Midi, Mini | DsRedEx | Cell By Date concept 1 with visual reporter | |
BBa_I719005 | pT7 | Midi | DsRedEx | Cell By Date concept 2 | ||
07 | BBa_F2620 | ptet-luxR-plux | XL1, Midi, Mini | DsRedEx | Infector Detector construct 1 with visual reporter | |
21 | BBa_J23039 | ptet-luxI | XL1, Midi | --- | AHL sender construct | |
24 | BBa_R0062 | plux | XL1, Midi | DsRedEx | Infector Detector construct 2 with visual reporter | |
25 | BBa_J37032 | plux-GFP | XL1, Maxi, Midi | --- | Infector Detector construct 2 | |
13 | BBa_B0015 | Double terminator | XL1, Maxi, Midi, Mini | --- | General part construction | |
17 | BBa_I13507 | RFP | XL1, Midi | --- | Not being used anymore | |
19 | BBa_B0034 | strong RBS | XL1 | --- | General part construction | |
23 | BBa_I13504 | GFP | XL1, Maxi, Midi | pT7 | Used to construct BBa_I719015 | |
BBa_I719??? | DsRed Express | Delayed | ptet, plux, pT7 | The Visual reporter | ||
BBa_I719??? | HrpR-HrpS | Ready | ??? | Hrp regulators | ||
BBa_I719??? | pHrpL | Ready | ??? | Hrp promoter | ||
BBa_I719??? | HrpV | Ready | ??? | Hrp inhibitor system | ||
Ligation Table
Status of parts ligated and their applications:
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low]
I | ID | Part | Description | Status | Parents | Application |
I | BBa_I719015 | pT7 & GFP | XL1, Maxi, Mini, BL21 | pT7 + BBa_I13504 | Cell By Date concept 2 | |
BBa_I719??? | DsRedEx & pSB1A3 | Paused | DsRedEx + BBa_P1010 | Biobrick Submission | ||
BBa_I719??? | pT7 & DsRedEx | Planned | pT7 + DsRedEx | Cell By Date concept 2 with visual reporter | ||
BBa_I719??? | ptet & DsRedEx | Planned | BBa_R0040 + DsRedEx | Cell By Date concept 1 with visual reporter | ||
BBa_I719??? | ptet-luxR-plux-DsRedEx | Planned | BBa_F2620 + DsRedEx | Infector Detector construct 1 with visual reporter | ||
BBa_I719??? | plux-DsRedEx | Planned | BBa_R0062 + DsRedEx | Infector Detector construct 2 with visual reporter | ||
Parts from the Registry
Physical information of biobricks:
Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced) [S - partially sequenced]
A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(T) | Location | Conc. (ng/ul) |
A | W | G | S | 02 | BBa_I13522 | GFP under tetracyclin promoter | pSB1A2 | AmpR | High | 937bp | 3016bp | Well 15H, Plate 2 | 500 |
A | W | G | 05 | BBa_R0040 | tetracyclin promoter | pSB1A2 | AmpR | High | 54bp | 2133bp | Well 7O, Plate 1 | 60 | |
A | W | G | S | 07 | BBa_F2620 | lux promoter, with luxR | pSB1A2 | AmpR | High | 1061bp | 3140bp | Well 6B, Plate 1 | 80 |
A | X | G | S | 11 | BBa_E7104 | T7 GFP | pSB1A2 | AmpR | High | 826bp | 2905bp | Well 13F, Plate 2 | 100 |
A | W | G | 13 | BBa_B0015 | Double terminator | pSB1AK3 | A+KR | High | 129bp | 3316bp | Well 1B, Plate 2 | 100/70 | |
A | G | 17 | BBa_I13507 | RFP | pSB1A2 | AmpR | High | 861bp | 2940bp | Well 16N, Plate 1 | 80 | ||
A | W | G | S | 18 | BBa_T9002 | GFP under lux promoter with LuxR | pSB1A3 | AmpR | High | 1945bp | 4102bp | Well 19B, Plate 2 | 460 |
A | W | 19 | BBa_B0034 | RBS | pSB1A2 | AmpR | High | 12bp | 2091bp | Well 3O, Plate 1 | -- | ||
A | G | 21 | BBa_J23039 | Constitutive luxI | pSB1A2 | AmpR | High | 860bp | 2939bp | Well 11K, Plate 3 | 140 | ||
A | W | G | S | 23 | BBa_I13504 | GFP | pSB1A2 | AmpR | High | 875bp | 2954bp | Well 16E, Plate 1 | 120/330 |
A | G | 24 | BBa_R0062 | lux promoter | pSB1A2 | AmpR | High | 55bp | 2134bp | Well 9G, Plate 1 | 90 | ||
A | G | 25 | BBa_J37032 | GFP under lux promoter | pSB1A2 | AmpR | High | 938bp | 3017bp | Well 8D, Plate 3 | 125/330 | ||
W | 30 | --- | T7 with GFP(strong) | --- | Kan? | ??? | --- | ??? | --- | 150 | |||
Our Parts
Physical information of our ligated parts:
Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)
Concentrations are given in ng/μl
A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(P) | Stock |
A | W | G | S | BBa_I791005 | T7 promoter | pSB1A2 | AmpR | High | 23bp | 2102bp | ||
A | W | G | S | BBa_I791015 | GFP under T7 promoter | pSB1A2 | AmpR | High | 906bp | 2985bp | ||
Part Graveyard
General information on parts we do not like...
A | W | ID | Name | Description | Plasmid | Size | Status | Notes |
A | W | 01 | BBa_E0040 | GFP | pSB1A2 | 720bp | XL1, Mini | Redundant |
A | 03 | BBa_J5528 | pBad-GFP | pSB2K3 | 2093bp | failed-XL1 | ara construct abandoned | |
A | 04 | BBa_J24677 | plux-GFP | pSB1A2 | 1787bp | XL1, Mini | Wrong GFP ligated | |
A | 06 | BBa_I0500 | pBad | pSB2K3 | 1210bp | XL1, Mini, Midi | ara construct abandoned | |
A | W | 08 | BBa_F1610 | luxI | pSB1AK3 | 798bp | XL1, Mini, Midi | Substitute part J23039 |
A | W | 09 | BBa_R0010 | lacI-plac | pSB1A2 | 200bp | XL1, Mini | Lac construct abandoned |
A | W | 10 | BBa_E1010 | RFP | pSB2K3 | 681bp | XL1, Mini | Substitute part I13507 |
A | 12 | BBa_S03550 | araC | pSB1AC3 | 958bp | XL1, Mini, Midi | araC construct abandoned | |
W | 14 | BBa_R0051 | pcI | pSB1A2 | 49bp | Biobrick | pcI construct abandoned | |
A | 15 | BBa_J07037 | cI-GFP | pSB2K3 | 912bp | XL1, Mini | No RBS present on this part | |
A | W | 16 | BBa_E0240 | GFP | pSB1A2 | 876bp | XL1, Midi | Redundant |
W | 20 | --- | AraC | pSB1AC3 | 1095bp | Planned | araC construct abandoned | |
A | 26 | BBa_S03119 | ptet-LuxR | pSB1A2 | 998bp | XL1, Midi | not used in design | |
A | 27 | pSB1A3 | pSB1A3 | pSB1A3 | 2157bp | failed-XL1 | biobrick not present | |
A | W | 28 | BBa_P1010 | pSB1A3 (ccd) | pSB1A3 | 0bp | failed-XL1 | Strain not available |
A | W | 29 | BBa_P1010 | pSB1A2 (ccd) | pSB1A2 | 0bp | --- | Strain not available |