Imperial/Wet Lab/Protocols

From 2007.igem.org

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<font size=2>'''Protein Purification'''</font> <br>
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<font size=2><font color=green>'''Protein Purification'''</font> <br>
* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and Results
* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and Results
* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and Results
* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and Results
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<font size=2>'''Fluorometer Testing'''</font> <br>
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<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and Results
* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and Results
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<font size=2>'''Calibration Curve'''</font> <br>
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<font size=2><font color=green>'''Calibration Curve'''</font> <br>
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and Results
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and Results
* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and Results
* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and Results
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<font size=2>'''Chassis Protocols'''</font> <br>
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<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
* ''Preparation S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
* ''Preparation S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
* ''Preparation S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
* ''Preparation S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
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<font size=2>'''pT7 Characterisation'''</font> <br>
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<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
*''pT7 in vitro:'' Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results]
*''pT7 in vitro:'' Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results]
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<font size=2>'''Cell by Date'''</font> <br>
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<font size=2><font color=green>'''Cell by Date'''</font>  
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Implementation<br>
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'''Implementation'''
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* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and  [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and  [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
* ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
* ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
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Testing/Validation<br>
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'''Testing/Validation<br>'''
*''Testing DNA Concentration in vitro'':
*''Testing DNA Concentration in vitro'':
* ''Operating Temperature Range'' :[[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
* ''Operating Temperature Range'' :[[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
*''Temperature Steps:'' Protocols and Results
*''Temperature Steps:'' Protocols and Results
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<font size=2>'''Infecter Detector'''</font> <br>
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<font size=2><font color=green>'''Infecter Detector'''</font> <br>
Implementation<br>
Implementation<br>
*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]

Revision as of 21:21, 22 October 2007


Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification

  • Purification of LuxR : Protocol and Results
  • Purification of GFPmut3b: Protocol and Results


Fluorometer Testing

  • Optimisation of Fluorometer: Protocol and Results


Calibration Curve

  • Calibration Curve for GFPmut3b: Protocol and Results
  • Degradation Curve for GFPmut3b :Protocol and Results


Chassis Protocols


pT7 Characterisation

  • pT7 in vitro: Protocols and Results

Cell by Date
Implementation

Testing/Validation

  • Testing DNA Concentration in vitro:
  • Operating Temperature Range :Protocol 1 and Protocol 2 and Results
  • Temperature Steps: Protocols and Results

Infecter Detector
Implementation

Testing/Validation

  • Testing of Purified LuxR :Protocol and Results
  • Characterization in vitro - AHL titration : Protocol and Results


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