Revision as of 22:03, 22 October 2007

Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments

Testing Experiments

Protein Purification

Purification of LuxR : Protocol and Results
Purification of GFPmut3b: Protocol and Results

Fluorometer Testing

Optimisation of Fluorometer: Protocol and Results

Calibration Curve

Calibration Curve for GFPmut3b: Protocol and Results
Degradation Curve for GFPmut3b :Protocol and Results

Chassis Protocols

Preparation S30 Cell Extract :Protocol
Preparation S12 Cell Extract :Protocol
Preparation of Reaction Mixture: Protocol
Vesicles Formation :Vesicles Formation

pT7 Characterisation

pT7 in vitro: Protocols and Results

Cell by Date
Implementation

In Vivo Testing: Protocols and Results
Initial In vitro Testing:Protocols and Results

Testing/Validation

Testing DNA Concentration in vitro: Protocol and Results
Operating Temperature Range :Protocol 1 and Protocol 2 and Results
Temperature Steps: Protocols and Results

Infecter Detector
Implementation

In vivo Testing: Protocol and Results
Initial In Vitro Testing: Protocol and Results

Testing/Validation

Testing of DNA concentration : Protocol and Results
Testing of Purified LuxR :Protocol and Results
Characterization in vitro - AHL titration : Protocol and Results

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 <font size=2><font color=green>'''Protein Purification'''</font> <br>
-* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [IGEM:Imperial/2007/Wet_Lab/results/Res1.5|Results]
+* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]
-* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.6|Results]]
+* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.6 Results]
 <br>
 <font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
-* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.1|Results]]<br>
+* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.1 Results]<br>
 <font size=2><font color=green>'''Calibration Curve'''</font> <br>
-* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.3|Results]]
+* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.3 Results]
-* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.4|Results]]
+* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.4 Results]
 <br>
 <font size=2><font color=green>'''Chassis Protocols'''</font> <br>
@@ Line 29: / Line 29: @@
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 <font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
-*''pT7 in vitro:'' Protocols and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.9|Results]]
+*''pT7 in vitro:'' Protocols and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.9 Results]
 <font size=2>'''Cell by Date'''</font>
 <br>

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Imperial/Wet Lab/Protocols

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Revision as of 22:03, 22 October 2007

Wet Lab: Protocols