Imperial/Wet Lab/Protocols

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= Wet Lab: Protocols =
= Wet Lab: Protocols =
Below is a databank of all the protocols that have been used in our projects.  
Below is a databank of all the protocols that have been used in our projects.  
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{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
|- style="background:#aabadd; color:Black" align="center"  
|- style="background:#aabadd; color:Black" align="center"  
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|| <font size=3>'''Fabrication Protocols'''</font>|| <font size=3>'''Testing Protocols'''</font>
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||<font size=2>'''General Experiments'''</font>||<font size=2>'''Testing Experiments'''</font>
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<font size=3>'''Construct Protocols'''</font> <br>
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<font size=2><font color=green>'''Protein Purification'''</font> <br>
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* [[Imperial/Wet_Lab/Protocols/DNA1.1|Cloning of DNA- MiniPrep]]
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* ''Purification of LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.5|Results]]  
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* [[Imperial/Wet_Lab/Protocols/DNA1.2|Cloning of DNA- MidiPrep]]
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* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.6|Results]]
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* [[Imperial/Wet_Lab/Protocols/DNA1.3|Cloning of DNA- MaxiPrep]]
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<font size=3>'''Protein Purification'''</font> <br>
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<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
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* [[Imperial/Wet_Lab/Protocols/Prot1.5|Purification of LuxR]]
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* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.1|Results]]<br>
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* [[Imperial/Wet_Lab/Protocols/Prot1.7|Testing of Purified LuxR]]
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<font size=2><font color=green>'''Calibration Curve'''</font> <br>
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/Construct_2 Results]
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* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.3|Results]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.6|Purification of GFPmut3b]]
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* ''Degradation Curve for GFPmut3b'':[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.4|Results]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.9|GFPmut3b Calibration Curve (Wrong, this is the recombinant GFP]]
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<font size=3>'''Fluorometer Testing'''</font> <br>
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<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
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* ''Reaction Mixture of Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.1|Optimisation of Fluorometer]]
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* ''Reaction Mixture of Homemade S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.4|Protocol]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_3.1 Results]
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* ''Recipe for S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation?? Wrong.]]
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* ''Recipe for S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]]
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* ''Testing of Homemade S30 Cell Extract with ID construct '':[[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1|Results]]<br>
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_1.1 Results]
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* ''Vesicles Formation'': [[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.3|Calibration Curve for DsRed express]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.4|Degradation Curve (For GFP? Non-existent)]]
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<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
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*''pT7 in vitro:'' [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.9|Results]]
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<font size=3>'''Chassis Protocols'''</font> <br>
 
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'''''E. coli'' Extracts'''<br>
 
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* [[Imperial/Wet_Lab/Protocols/CE1.1|Preparation of S30 ''E. coli'' extract]]
 
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* [[Imperial/Wet_Lab/Protocols/CE1.2|Preparation of S12 ''E. coli'' extract]]
 
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* [[Imperial/Wet_Lab/Protocols/Protocols/CE1.3|Preparation of reaction mixture]]
 
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'''Vesicles Formation'''<br>
 
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* [[Imperial/Wet_Lab/Protocols/VF1.1|Vesicles Formation]]
 
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* 2
 
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<font size=3>'''Cell by Date'''</font> <br>
 
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'''Implementation'''<br>
 
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* [[Imperial/Wet_Lab/Protocols/CBD1.1|Initial Testing for DNA constructs of CBD In Vivo]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
 
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* [[Imperial/Wet_Lab/Protocols/CBD1.2|Initial Testing for DNA constructs of CBD In Vitro (Chassis)]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
 
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'''Testing/Validation'''<br>
 
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* [[Imperial/Wet_Lab/Protocols/CBD2.1|Operating Temperature Range (Initial Tests)]]
 
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* [[Imperial/Wet_Lab/Protocols/CBD2.2|Operating Temperature Range (Refined Tests)]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.1.1 Results + ones for initial operating range]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results for pT7]
 
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* [[Imperial/Wet_Lab/Protocols/Prot1.8|Detemining System Capabilities - DNA concentration]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
 
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* Packaging protocol?? Results are in OWW.
 
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* Steps protocol?? Results are in OWW.
 
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<font size=2>'''Cell by Date'''</font> <br>
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<font color=green>'''Implementation'''</font><br>
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* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.1|Results]]
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* ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.2|Results]]
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<font color=green>'''Testing/Validation<br>'''</font>
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*''Testing DNA Concentration in vitro'': [[Imperial/Wet_Lab/Protocols/CBD1.3|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
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* ''Isothermal experiments'': [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results]]
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*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.3|Results]]
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*''Packaging experments:'' [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.1|Results]]
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<font size=3>'''Infector Detector'''</font> <br>
 
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'''Implementation'''<br>
 
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* [[Imperial/Wet_Lab/Protocols/ID1.1|Initial Testing for DNA constructs of ID In Vivo]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
 
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* [[Imperial/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro (Chassis)]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]
 
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<font size=2>'''Infector Detector'''</font> <br>
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<font color=green>'''Implementation<br>'''</font>
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*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.1|Results]]
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* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.2|Results]]
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<font color=green>'''Testing/Validation<br>'''</font>
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*''Testing of DNA concentration'' : [[Imperial/Wet_Lab/Protocols/ID2.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID2.1|Results]]
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* ''Testing of Purified LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.7|Results]]
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* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID3.1|Results]]
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'''Testing/Validation'''<br>
 
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* [[Imperial/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.2.1 Results]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.2.3 Results]
 
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* [[Imperial/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Biofilm_Detector/TestingValidation Results]
 
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* [[Imperial/Wet_Lab/Protocols/ID3.1|Final AHL Testing (Standardized)]]
 
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<center>| [[Imperial/Wet_Lab| Wet Lab >>]]</center>
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Latest revision as of 02:15, 27 October 2007




Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols

  • Reaction Mixture of Commercially available S30 Cell Extract :Protocol
  • Reaction Mixture of Homemade S30 Cell Extract :Protocol
  • Recipe for S30 Cell Extract :Protocol
  • Recipe for S12 Cell Extract :Protocol
  • Testing of Homemade S30 Cell Extract with ID construct :Protocol and Results
  • Vesicles Formation: Protocol


pT7 Characterisation


Cell by Date
Implementation

Testing/Validation


Infector Detector
Implementation

Testing/Validation



| Wet Lab >>

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