From 2007.igem.org
Wet Lab: Protocols
Below is a databank of all the protocols that have been used in our projects.
General Experiments | Testing Experiments
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Protein Purification
- Purification of LuxR : Protocol and Results
- Purification of GFPmut3b: Protocol and Results
Fluorometer Testing
- Optimisation of Fluorometer: Protocol and Results
Calibration Curve
- Calibration Curve for GFPmut3b: Protocol and Results
- Degradation Curve for GFPmut3b :Protocol and Results
Chassis Protocols
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pT7 Characterisation
- pT7 in vitro: Protocols and Results
Cell by Date
Implementation
Testing/Validation
- Testing DNA Concentration in vitro:
- Operating Temperature Range :Protocol 1 and Protocol 2 and Results
- Temperature Steps: Protocols and Results
Infecter Detector
Implementation
Testing/Validation
- Testing of Purified LuxR :Protocol and Results
- Characterization in vitro - AHL titration : Protocol and Results
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