From 2007.igem.org

• Home
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  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
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  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
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• Cell by Date
  • Introduction
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  • Design
  • Modelling
  • Implementation
  • Testing/Validation
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• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
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  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments

Testing Experiments

Protein Purification Purification of LuxR : Protocol and Results Purification of GFPmut3b: Protocol and Results Fluorometer Testing Optimisation of Fluorometer: Protocol and Results Calibration Curve Calibration Curve for GFPmut3b: Protocol and Results Degradation Curve for GFPmut3b :Protocol and Results Chassis Protocols Preparation S30 Cell Extract :Protocol Preparation S12 Cell Extract :Protocol Preparation of Reaction Mixture: Protocol Vesicles Formation :Vesicles Formation

pT7 Characterisation pT7 in vitro: Protocols and Results Cell by Date Implementation In Vivo Testing: Protocols and Results Initial In vitro Testing:Protocols and Results Testing/Validation Testing DNA Concentration in vitro: Operating Temperature Range :Protocol 1 and Protocol 2 and Results Temperature Steps: Protocols and Results Infecter Detector Implementation In vivo Testing: Protocol and Results Initial In Vitro Testing: Protocol and Results Testing/Validation Testing of Purified LuxR :Protocol and Results Characterization in vitro - AHL titration : Protocol and Results