Imperial/Wet Lab/Results/CBD2.1
From 2007.igem.org
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*Determine a suitable sampling technique which disrupts the reaction to a very minimum | *Determine a suitable sampling technique which disrupts the reaction to a very minimum | ||
<br>'''??-??-2007''' | <br>'''??-??-2007''' | ||
| + | |||
| + | ==Aims== | ||
| + | To determine if the of [http://partsregistry.org/Part:BBa_T9002 '''pTet-LuxR-pLux-GFPmut3b'''] construct works ''in vivo''. | ||
| + | |||
| + | |||
| + | ==Materials and Methods== | ||
| + | See [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] page | ||
| + | <br> | ||
| + | Tested on (Insert link) | ||
| + | |||
| + | ==Results== | ||
| + | {|align="center" | ||
| + | | width="600px"|<br>[[Image:|thumb|600px|Fig.1.1:pTet-LuxR-pLux-GFPmut3b ''in vivo'' - ]] | ||
| + | |} | ||
| + | |||
| + | {|align="center" | ||
| + | | width="600px"|<br>[[Image:.PNG|thumb|600px|]] | ||
| + | |} | ||
| + | |||
| + | '''Controls:''' | ||
| + | *Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually) | ||
| + | |||
| + | '''Constants:''' | ||
| + | *Temperature - 37°C | ||
| + | *Volume of Cells sampled - 60µl | ||
| + | |||
| + | |||
| + | '''Raw Data''' | ||
| + | *[[Media:.xls|Raw Data excel file]] | ||
| + | |||
| + | ==Discussion== | ||
| + | |||
| + | |||
| + | ==Conclusion== | ||
Revision as of 23:31, 25 October 2007
Wet Lab: ResultsTest for optimum packaging and sampling techniques
pTet-GFP
Aim
- Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
- Determine a suitable sampling technique which disrupts the reaction to a very minimum
??-??-2007
Aims
To determine if the of pTet-LuxR-pLux-GFPmut3b construct works in vivo.
Materials and Methods
See Protocols page
Tested on (Insert link)
Results
[[Image:|thumb|600px|Fig.1.1:pTet-LuxR-pLux-GFPmut3b in vivo - ]] |
Controls:
- Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)
Constants:
- Temperature - 37°C
- Volume of Cells sampled - 60µl
Raw Data
