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	<br>'''??-??-2007'''		<br>'''??-??-2007'''
-	==Aims==
-	To determine if the of [http://partsregistry.org/Part:BBa_T9002 '''pTet-LuxR-pLux-GFPmut3b'''] construct works ''in vivo''.

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Revision as of 23:34, 25 October 2007

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Wet Lab: ResultsTest for optimum packaging and sampling techniques

pTet-GFP

Aim

• Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
• Determine a suitable sampling technique which disrupts the reaction to a very minimum

??-??-2007

Materials and Methods

See Protocols page
Tested on (Insert link)

Results

[[Image:|thumb|600px|Fig.1.1:pTet-LuxR-pLux-GFPmut3b in vivo - ]]

File:.PNG

Controls:

• Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)

Constants:

• Temperature - 37°C
• Volume of Cells sampled - 60µl

Raw Data

• Raw Data excel file

Discussion

Conclusion