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Revision as of 00:05, 26 October 2007

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Wet Lab: ResultsTest for optimum packaging and sampling techniques

pTet-GFP

Aim

• Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
• Determine a suitable sampling technique which disrupts the reaction to a very minimum

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Materials and Methods

See Protocols page
Tested on (Insert link)

Results

Fig.1.1: pTet-GFPmut3b - fluorescence output for various packaging and sampling techniques, over a period of 6 hours

Controls:

• Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)

Constants:

• Temperature - 37°C
• Volume of Cells sampled - 60µl

Raw Data

• Raw Data excel file

Discussion

Conclusion