Imperial/Wet Lab/Results/CBD2.1
From 2007.igem.org
m (→Discussion) |
m (→Discussion) |
||
| Line 36: | Line 36: | ||
==Discussion== | ==Discussion== | ||
| - | It can be seen from the results that the fluorescence is the highest for the plate with multiple layers of tape. Thus putting extra tape on the wells | + | It can be seen from the results that the fluorescence is the highest for the plate with multiple layers of tape. Thus putting extra tape on the wells chnages the fluorescence of the system. As it has been seen before evaporation of samples results in greater fluorescence, it means there is more evaporation in the multi layered plate.?? |
For the pipetting sample, two readings were taken for each data point. One before and one after pipetting the mixture. It can be seen that pipetting disrupts the reaction as fluorescence after pipetting increase to a very high value. | For the pipetting sample, two readings were taken for each data point. One before and one after pipetting the mixture. It can be seen that pipetting disrupts the reaction as fluorescence after pipetting increase to a very high value. | ||
| - | The PCR method seems to have the least fluorescnce out of all the samples. | + | The PCR method seems to have the least fluorescnce out of all the samples. As PCR methiod requires pipetting and centrifuging (not tested here), there will be a lot of disruption in the reaction. |
==Conclusion== | ==Conclusion== | ||
Revision as of 00:34, 26 October 2007
Wet Lab: ResultsTest for optimum packaging and sampling techniques
pTet-GFP
Aim
- Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
- Determine a suitable sampling technique which disrupts the reaction to a very minimum
??-??-2007
Materials and Methods
See Protocols page
Tested on (Insert link)
Results
 |
Controls:
- Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)
Constants:
- Temperature - 37°C
- Volume of Cells sampled - 60µl
Raw Data
Discussion
It can be seen from the results that the fluorescence is the highest for the plate with multiple layers of tape. Thus putting extra tape on the wells chnages the fluorescence of the system. As it has been seen before evaporation of samples results in greater fluorescence, it means there is more evaporation in the multi layered plate.??
For the pipetting sample, two readings were taken for each data point. One before and one after pipetting the mixture. It can be seen that pipetting disrupts the reaction as fluorescence after pipetting increase to a very high value.
The PCR method seems to have the least fluorescnce out of all the samples. As PCR methiod requires pipetting and centrifuging (not tested here), there will be a lot of disruption in the reaction.
