Imperial/Wet Lab/Results/CBD2.1

From 2007.igem.org

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*Determine a suitable sampling technique which disrupts the reaction to a very minimum
*Determine a suitable sampling technique which disrupts the reaction to a very minimum
<br>'''??-??-2007'''
<br>'''??-??-2007'''
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==Aims==
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To determine if the of [http://partsregistry.org/Part:BBa_T9002 '''pTet-LuxR-pLux-GFPmut3b'''] construct works ''in vivo''.
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==Materials and Methods==
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See [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] page
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<br>
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Tested on  (Insert link)
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==Results==
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{|align="center"
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| width="600px"|<br>[[Image:|thumb|600px|Fig.1.1:pTet-LuxR-pLux-GFPmut3b ''in vivo'' - ]]
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|}
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{|align="center"
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| width="600px"|<br>[[Image:.PNG|thumb|600px|]]
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|}
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'''Controls:'''
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*Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)
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'''Constants:'''
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*Temperature -  37&deg;C
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*Volume of Cells sampled - 60&micro;l
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'''Raw Data'''
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*[[Media:.xls|Raw Data excel file]]
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==Discussion==
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==Conclusion==

Revision as of 23:31, 25 October 2007


Wet Lab: ResultsTest for optimum packaging and sampling techniques

[http://partsregistry.org/Part:BBa_I13522 pTet-GFP]

Aim

  • Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
  • Determine a suitable sampling technique which disrupts the reaction to a very minimum


??-??-2007

Aims

To determine if the of [http://partsregistry.org/Part:BBa_T9002 pTet-LuxR-pLux-GFPmut3b] construct works in vivo.


Materials and Methods

See Protocols page
Tested on (Insert link)

Results


[[Image:|thumb|600px|Fig.1.1:pTet-LuxR-pLux-GFPmut3b in vivo - ]]

Controls:

  • Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)

Constants:

  • Temperature - 37°C
  • Volume of Cells sampled - 60µl


Raw Data

Discussion

Conclusion