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Wet Lab: ResultsTest for optimum packaging and sampling techniques

pTet-GFP

Aim

• Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
• Determine a suitable sampling technique which disrupts the reaction to a very minimum

??-??-2007

Aims

To determine if the of pTet-LuxR-pLux-GFPmut3b construct works in vivo.

Materials and Methods

See Protocols page
Tested on (Insert link)

Results

[[Image:|thumb|600px|Fig.1.1:pTet-LuxR-pLux-GFPmut3b in vivo - ]]

File:.PNG

Controls:

• Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)

Constants:

• Temperature - 37°C
• Volume of Cells sampled - 60µl

Raw Data

• Raw Data excel file

Discussion

Conclusion