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Wet Lab: ResultsTest for optimum packaging and sampling techniques

pTet-GFP

Aim

• Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
• Determine a suitable sampling technique which disrupts the reaction to a very minimum

??-??-2007

Materials and Methods

See Protocols page
Tested on (Insert link)

Results

Fig.1.1: pTet-GFPmut3b - fluorescence output for various packaging and sampling techniques, over a period of 6 hours

Controls:

• Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)

Constants:

• Temperature - 37°C
• Volume of Cells sampled - 60µl

Raw Data

• Raw Data excel file

Discussion

It can be seen from the results that the fluorescence is the highest for the plate with multiple layers of tape. Thus putting extra tape on the wells changes the fluorescence of the system ????

For the pipetting sample, two readings were taken for each data point. One before and one after pipetting the mixture. It can be seen that pipetting disrupts the reaction as fluorescence after pipetting increase to a very high value.

The PCR method seems to have the least fluorescnce out of all the samples.

Conclusion