Melbourne/4 July 07 Digest
From 2007.igem.org
(Difference between revisions)
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====Aim: ==== | ====Aim: ==== | ||
- | *Confirmation of contents of minipreped plasmids. | + | *Confirmation of contents of minipreped plasmids from kit and elsewhere. |
====Method:==== | ====Method:==== | ||
- | # | + | # Made up 25 Ampicillin plates using 400mL LB agar.(Using 100mg/ml Ampicillin stock from Gooley Lab). |
- | # | + | # Make up Kanamycin plates. |
+ | #[[Melbourne/IGEM 2007 kit|Resuspended the following from Registry plates]]. | ||
+ | #[[Melb:Transformation Protocol|Transformed]] into Joe's competent DH5alpha cells. | ||
+ | # Streak the cells onto plates. | ||
+ | #[[Melb:Growing up cells|Prepared]] in Amp LB (100ug/mL) or Kan LB (50mg/mL) as appropriate. | ||
+ | #[[Melb:Miniprep protocol|Minipreped]] the cultures grown overnight after putting aside 1mL(sterile) for glycerol stocks. | ||
+ | #[[Melbourne/Glycerol Stocks|made glycerol stocks]]. | ||
+ | #[[Melbourne/Preparing an agarose gel|Prepared an agarose gel]] with 1xTAE buffer. | ||
+ | # Digest | ||
+ | #[[Melbourne/Loading a DNA gel|Loaded]] 20uL of digest samples from 6/7 in the following lane order. | ||
====Results:==== | ====Results:==== |
Revision as of 07:47, 10 July 2007
<List of experiments> <Back to team home page>
Contents |
Dates:
- Method Documentation Comenced:1 July 2007
- Method Documentation Completed:
- Author:
- Experiment Commenced:
- Experiment Completed:10 July 2007
- Write up Completed:
Aim:
- Confirmation of contents of minipreped plasmids from kit and elsewhere.
Method:
- Made up 25 Ampicillin plates using 400mL LB agar.(Using 100mg/ml Ampicillin stock from Gooley Lab).
- Make up Kanamycin plates.
- Resuspended the following from Registry plates.
- Transformed into Joe's competent DH5alpha cells.
- Streak the cells onto plates.
- Prepared in Amp LB (100ug/mL) or Kan LB (50mg/mL) as appropriate.
- Minipreped the cultures grown overnight after putting aside 1mL(sterile) for glycerol stocks.
- made glycerol stocks.
- Prepared an agarose gel with 1xTAE buffer.
- Digest
- Loaded 20uL of digest samples from 6/7 in the following lane order.
Results:
- Jottings:
- Gells:
- Pictures: