From 2007.igem.org
Wet Lab: Protocols
Below is a databank of all the protocols that have been used in our projects.
| General Experiments | Testing Experiments
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Protein Purification
Fluorometer Testing
Calibration Curve
Chassis Protocols
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pT7 Characterisation
Cell by Date
Implementation
Testing/Validation
- Testing DNA Concentration in vitro: Protocols and Results
- Operating Temperature Range: Protocol 1 & Protocol 2 and Results 1 & [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
- Temperature Steps: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]
Infecter Detector
Implementation
- In vivo Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]
- Initial In Vitro Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]
Testing/Validation
- Testing of DNA concentration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res2.1 Results]
- Testing of Purified LuxR: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]
- Characterization in vitro - AHL titration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 Results]
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