Melbourne/Primary Reagents & disposables

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[[Melbourne|<Back to team home page>]]    [[Melb:Primary Reagent Name:T|<Template>]]
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[[Melbourne|<Back to team home page>]]    [[Melbourne/Primary Reagent Name:T|<Template>]]
==Primary Reagents==
==Primary Reagents==
===Kits===
===Kits===
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*[[Melb:Miniprep kit|Miniprep kit]]  
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*[[Melbourne/IGEM 2007 kit|IGEM 2007 kit]]
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*Gel clean-up system
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*[[Melbourne/Miniprep kit|Miniprep kit]]  
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*[[Melbourne/Gel clean up kit|Gel clean up kit]]
*Site directed mutagenesis (stratagene)
*Site directed mutagenesis (stratagene)
**Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
**Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
**Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
**Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
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**QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers  80% >8Kb
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**[[Melbourne QuickchangeXL|QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers  80% >8Kb]]
*Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
*Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
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===Cells===
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*[[Melbourne pBluescriptKS|pBluescriptKS+]]
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*[[Melbourne DH5a|DH5a super competant cells]]
===Enzymes===
===Enzymes===
*[[Melbourne/primary Restriction enzymes]]
*[[Melbourne/primary Restriction enzymes]]
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** NotI,
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*[[Melbourne/primary Ligase|T4 Ligase]]
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**EcoRI,
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*[[Melbourne/primary 10X Ligase buffer|T4 Ligase buffer 10X]]
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**XbaI,
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*[[Melbourne/primary 2X Ligase buffer|T4 Ligase buffer 2X]]
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**HindIII,
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**SpeI,
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*[[Melbourne/primary Ligase|Ligase]]
+
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*[[Melbourne/primary competant DH5a|DH5a Competent cells]]
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*[[Melbourne/primary DNA marker|DNA ladder]]
*[[Melbourne/primary DNA marker|DNA ladder]]
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*[[Melbourne/primary phosphatase|phospatase]]
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*[[Melbourne/primary BSA|BSA]]
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*[[Melbourne/primary GoTaq|GoTaq]]
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*[[Melbourne/primary vf2|vf2 oligonucleotide]]
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*[[Melbourne/primary vR|vR oligonucleotide]]
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===Antibiotics===
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*[[Melbourne/primary AMP|Ampicilian]]
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*[[Melbourne/primary Kan|Kanamycin]]
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*[[Melbourne/primary Chloroamphenicol|Chloroamphenicol]]
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*[[Melbourne/primary Gen|Gentimycin]]
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 +
===Chemicals===
===Chemicals===
*[[Melbourne/primary agar|Agar]]
*[[Melbourne/primary agar|Agar]]
*[[Melbourne/primary Agarose|Agarose]]
*[[Melbourne/primary Agarose|Agarose]]
*[[Melbourne/primary EB|Ethidium Bromide]]
*[[Melbourne/primary EB|Ethidium Bromide]]
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*[[Melbourne/primary EDTA|EDTA]]*[[Melbourne/primary glycerol|Glycerol]]
+
*[[Melbourne/primary EDTA|EDTA]]
 +
*[[Melbourne/primary glycerol|Glycerol]]
*[[Melbourne/primary dna loading|Loading dye for DNA gel]]
*[[Melbourne/primary dna loading|Loading dye for DNA gel]]
*[[Melbourne/primary Nacl|NaCl]]
*[[Melbourne/primary Nacl|NaCl]]
*[[Melbourne/primary Tris|TRIS]]
*[[Melbourne/primary Tris|TRIS]]
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*[[Melbourne/primary IPTG|IPTG]]
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*[[Melbourne/primary Xgal|Xgal]]
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*[[Melbourne/primary Bacto-tryptone|Bacto-tryptone]]
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*[[Melbourne/primary Yeast Extract|Yeast Extract]]
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*[[Melbourne/primary NaOH|NaOH]]
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*[[Melbourne/primary NZ amine|NZ amine]]
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*[[Melbourne/primary Bacto-yeast Extract|Bacto-yeast Extract]]
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*[[Melbourne/primary Ethanol|Ethanol]]
===Other===
===Other===
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*[[Melbourne/primary DNA Loading dye|DNA Loading Dye]]
*[[Melbourne/primary ice|ice]]
*[[Melbourne/primary ice|ice]]
*[[Melbourne/primary milliq|milliQ water]]
*[[Melbourne/primary milliq|milliQ water]]
*[[Melbourne/primary distilled|Distilled water]]
*[[Melbourne/primary distilled|Distilled water]]
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*[[Melbourne/primary Liquid N2|Liquid Nitrogen]]
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*[[Melbourne/primary Solid CO2|Dry Ice]]
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==Disposables==
==Disposables==
*[[Melbourne/primary Parrafilm|Parrafilm]]
*[[Melbourne/primary Parrafilm|Parrafilm]]
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===TUBES:===
===TUBES:===
*[[Melbourne/primary falcon|Falcon: 10ml, 50ml]]
*[[Melbourne/primary falcon|Falcon: 10ml, 50ml]]
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*microcentrifuge: 1.7ml
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*[[Melbourne/1.7ml microcentrifuge|microcentrifuge: 1.7ml]]
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*PCR
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*[[Melbourne/0.5ml microcentrifuge|microcentrifuge: 0.6ml]]
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*[[Melbourne/Screw Top tubes|Screw Top tubes]]

Latest revision as of 11:48, 8 October 2007

<Back to team home page> <Template>

Contents

Primary Reagents

Kits

Cells

Enzymes

Antibiotics


Chemicals

Other

Disposables

TUBES: