Imperial/Infector Detector/Implementation
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+ | <link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /> | ||
+ | |||
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+ | <div id="tabs"> | ||
+ | <ul> | ||
+ | <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction" title=""><span>Introduction</span></a></li> | ||
+ | <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Specification" title=""><span>Specifications</span></a></li> | ||
+ | <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Design" title=""><span>Design</span></a></li> | ||
+ | <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Modelling" title=""><span>Modelling</span></a></li> | ||
+ | <li><a class="current" href="https://2007.igem.org/Imperial/Infector_Detector/Implementation" title=""><span>Implementation</span></a></li> | ||
+ | <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Testing" title=""><span>Testing</span></a></li> | ||
+ | <li><a href="https://2007.igem.org/Imperial/Infector_Detector/F2620 Comparison" title=""><span>F2620</span></a></li> | ||
+ | <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Conclusion" title=""><span>Conclusion</span></a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <hr /> | ||
+ | <br clear="all"> | ||
+ | </html> | ||
__NOTOC__ | __NOTOC__ | ||
+ | = Infector Detector: Implementation = | ||
+ | |||
+ | |||
+ | {|border=1 width=100% align="center" cellpadding="10px" | ||
+ | |- | ||
+ | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation''' </center> | ||
+ | |- align="center" | ||
+ | |wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' | ||
+ | |width="40%" align="left" | [[Image:IC07_design_ID.png|center|370px]] | ||
+ | |width="40%" align="left" | [[Image:IC07_design_ID-con2.png|center|370px]] | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
+ | |width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. <br />[[Image:Imperial ID Design ID1.png|center|]] | ||
+ | |width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. <br /> [[Image:Imperial_ID_Design_ID2.png|center]] | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
+ | |width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]] | ||
+ | |width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]] | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
+ | |width="40%"| Not Required | ||
+ | |width="40%"| Not Required | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
- | == | + | |width="40%"| Not Required |
+ | |width="40%"| [[Image:Purification_of_LuxR.PNG|center|thumb|200px|Purification of LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results])]] | ||
+ | |- | ||
+ | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
+ | |width="40%"| [[Image:IC_2007_Plux_in_vivo.PNG|center|350px]]<br /> | ||
+ | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.1 Results]) | ||
+ | * '''Sequencing of Construct''': Partially Sequenced | ||
+ | |width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br /> | ||
+ | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results]) | ||
+ | * '''Sequencing of Construct''': Completed | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | ||
+ | |width="40%" style="background-color:#b8efb0"| Ready for Testing | ||
+ | |width="40%" style="background-color:#ddb99e"| Aborted. <br /> LuxR protein not functional. | ||
+ | |} | ||
+ | <br clear="all"> | ||
- | |||
- | (Quality Control | + | {|border=1 width=100% align="center" cellpadding="10px" |
+ | |- | ||
+ | |colspan="3" style="background-color:#eeeeff"| <center>'''Chassis Construct Implementation'''</center> | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Chassis Design''' | ||
+ | |colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]] | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
+ | |width="40%"| Possibility of making Homemade S30 Cell Extract. | ||
+ | |width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
+ | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]) | ||
+ | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | ||
+ | |- | ||
+ | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
+ | |width="40%"| | ||
+ | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]]) | ||
+ | |width="40%"| | ||
+ | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.2 Results]) | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' | ||
+ | |width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | ||
+ | |width="40%" style="background-color:#b8efb0"| Ready for Testing | ||
+ | |} | ||
+ | <br clear="all"> | ||
- | |||
- | + | <center> [https://2007.igem.org/Imperial/Infector_Detector/Modelling << Modelling] | Implementation | [https://2007.igem.org/Imperial/Infector_Detector/Testing Testing/Validation >>] | |
+ | </center> |
Latest revision as of 02:33, 27 October 2007
Infector Detector: Implementation
| ||
Chassis Design | ![]() | |
Stage 1: Availability ![]() | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. |
Stage 2: Preparation ![]() | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control ![]() | ||
Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |