Imperial/Infector Detector/Implementation
From 2007.igem.org
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<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /> | <link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /> | ||
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|width="40%"| Not Required | |width="40%"| Not Required | ||
| - | |width="40%"| [[Image:Purification_of_LuxR.PNG|center| | + | |width="40%"| [[Image:Purification_of_LuxR.PNG|center|thumb|200px|Purification of LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results])]] |
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
|width="40%"| [[Image:IC_2007_Plux_in_vivo.PNG|center|350px]]<br /> | |width="40%"| [[Image:IC_2007_Plux_in_vivo.PNG|center|350px]]<br /> | ||
* '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.1 Results]) | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.1 Results]) | ||
| - | * '''Sequencing of Construct''': | + | * '''Sequencing of Construct''': Partially Sequenced |
|width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br /> | |width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br /> | ||
* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results]) | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results]) | ||
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in vitro''''': Failed ([[Protocol and Results) | + | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]]) |
|width="40%"| | |width="40%"| | ||
* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.2 Results]) | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.2 Results]) | ||
Latest revision as of 02:33, 27 October 2007
Infector Detector: Implementation
| | ||
| Construct Design |  |  |
Stage 1: Availability 
| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry.  | Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry.  |
| Stage 2: Cloning / Amplification
|  |  |
| Stage 3: Ligation
| Not Required | Not Required |
| Stage 4: Purification of Activator Proteins
| Not Required | |
| Stage 5: Quality Control
|  |  |
| Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
| | ||
| Chassis Design | 
| |
| Stage 1: Availability
| Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. |
| Stage 2: Preparation
| Preparation: Done (Protocol) | Preparation: Done (Protocol) |
| Stage 3: Quality Control
| ||
| Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |
