Imperial/Infector Detector/Implementation
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{|border=1 width=100% align="center" cellpadding="10px" | {|border=1 width=100% align="center" cellpadding="10px" | ||
|- | |- | ||
- | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center> | + | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation''' </center> |
+ | |- | ||
+ | |colspan="3" style="background-color:#eeeeff"|[[Image:IC07_design_ID.png|center|500px]] | ||
|- align="center" | |- align="center" | ||
|wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' | |wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' | ||
- | |width="40%" align="left" | | + | |width="40%" align="left" | [[Image:BB_c1.png|center|300px]] |
- | |width="40%" align="left" | | + | |width="40%" align="left" | [[Image:BB_c2.png|center|300px]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
- | |width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. | + | |width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. <br />[[Image:Imperial ID Design ID1.png|center|]] |
- | |width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. | + | |width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. <br /> [[Image:Imperial_ID_Design_ID2.png|center]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] |
Revision as of 09:17, 25 October 2007
Infector Detector: Implementation
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Construct Design | ||
Stage 1: Availability | Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. | Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. |
Stage 2: Cloning / Amplification | ||
Stage 3: Ligation | Not Required | Not Required |
Stage 4: Purification of Activator Proteins | Not Required | LuxR protein (Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]) |
Stage 5: Quality Control |
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Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
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Chassis Design | ||
Stage 1: Availability | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. |
Stage 2: Preparation | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control |
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Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |