Imperial/Infector Detector/Implementation
From 2007.igem.org
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|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | ||
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- | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' | + | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| | |width="40%"| | ||
* '''Testing ''in vitro''''': Passed (Protocol and Results) | * '''Testing ''in vitro''''': Passed (Protocol and Results) | ||
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* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]) | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]) | ||
|- align="center" | |- align="center" | ||
- | |width="20%" style="background-color:#ddeeff"| '''Stage | + | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' |
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | |width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | ||
|width="40%" style="background-color:#b8efb0"| Ready for Testing | |width="40%" style="background-color:#b8efb0"| Ready for Testing |
Revision as of 14:28, 24 October 2007
Infector Detector: Implementation
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Construct Design | Concept 1 | Concept 2 |
Stage 1: Availability | Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. | Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. |
Stage 2: Cloning / Amplification | ||
Stage 3: Ligation | Not Required | Not Required |
Stage 4: Purification of Activator Proteins | Not Required | LuxR protein (Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]) |
Stage 5: Quality Control |
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Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
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Chassis Design | ||
Stage 1: Availability | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. |
Stage 2: Preparation | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control |
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Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |